Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

Mfr.No.

CSB-E05105h

Sensitivity

10 ng/mL

Detection Range

20 ng/mL-4000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Dehydroepiandrosterone sulfate,DHEA-S

Recovery

The recovery of human TNFsR-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	101	103-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-105
1:2	Average %	91
	Range %	86-98
1:4	Average %	91
	Range %	88-96
1:8	Average %	101
	Range %	96-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	DHEA-S

Storage	Store at 2-8°C. Please refer to protocol.

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