Human Dehydroepiandrosterone,DHEA ELISA Kit

Mfr.No.

CSB-E08226h

Sensitivity

0.05 ng/mL

Detection Range

0.1 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Dehydroepiandrosterone,DHEA

Recovery

The recovery of human F11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-106
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human F11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	82-88
1:2000	Average %	110
	Range %	107-113
1:4000	Average %	91
	Range %	85-97
1:8000	Average %	92
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	DHEA

Storage	Store at 2-8°C. Please refer to protocol.

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