Matrices listed below were spiked with certain level of DEFB129 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFB129 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
88-102
94
heparin plasma(n=5)
90-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFB129 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
DEFB129 ELISA Kit allows for the in vitro quantitative determination of DEFB129 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human DEFB129(Beta-defensin 129) ELISA Kit