Human DEFa3(Defensin Alpha 3, Neutrophil Specific) ELISA Kit

Mfr.No.

ELK3141

Alternative Names

HNP3; DEF3; HNP-2; Neutrophil defensin 2

Reactivity

Human

Detection Range

0.47-30 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant DEFa3 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFa3 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	86-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFa3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	81-93%	89-104%	78-96%	79-98%
EDTA plasma(n=5)	93-102%	88-97%	79-92%	81-97%
Heparin plasma(n=5)	87-95%	82-94%	95-102%	87-103%

Typical Data

<table> <tbody><tr> <th>Concentration (ng/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>30.00</td> <td>2.154</td> <td>2.071</td> </tr> <tr> <td>15.00</td> <td>1.717</td> <td>1.634</td> </tr> <tr> <td>7.50</td> <td>1.185</td> <td>1.102</td> </tr> <tr> <td>3.75</td> <td>0.896</td> <td>0.813</td> </tr> <tr> <td>1.88</td> <td>0.539</td> <td>0.456</td> </tr> <tr> <td>0.94</td> <td>0.363</td> <td>0.280</td> </tr> <tr> <td>0.47</td> <td>0.232</td> <td>0.149</td> </tr> <tr> <td>0.00</td> <td>0.083</td> <td>0.000</td> </tr> </tbody></table>

Sensitivity

0.21 ng/mL

Limit Of Detection

30 ng/mL

UniProt ID	P59666
Antigen	DEFa3
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEFa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEFa3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Infection immunity;
Protocol	<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3141-1.pdf" target="_blank">Datasheet</a>
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203154	ELK3141	48T	$320		Add to Cart
BHE15203154	ELK3141	96T	$458		Add to Cart

Human DEFa3(Defensin Alpha 3, Neutrophil Specific) ELISA Kit

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