Human Dedicator of Cytokinesis Protein 1

Mfr.No.

E5173Hu

Sensitivity

9.72ng/L

Detection Range

20-6000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

180 kDa protein downstream of CRK;Dedicator of cytokinesis protein 1;DOCK 1;DOCK1;DOCK180

Molecular Weight

215346

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5173Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	254.4	18.51	7.3
Intra-Assay	2	18	560.6	35.49	6.3
Intra-Assay	3	18	1095	54.14	4.9

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed: 19004829). Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed: 8657152).

Protocol

These standard curves of E5173Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.157	2.139	2.098
	2.122
1600ng/L	1.55	1.586	1.544
	1.622
800ng/L	0.966	0.966	0.925
	0.967
400ng/L	0.779	0.735	0.694
	0.692
200ng/L	0.428	0.445	0.404
	0.462
0ng/L	0.031	0.041	0
	0.052

Gene ID	1793
UniProt ID	Q14185

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106536	E5173Hu	48T	$320		Add to Cart
BHE12106536	E5173Hu	96T	$458		Add to Cart

Human Dedicator of Cytokinesis Protein 1 - DOCK1 ELISA Kit

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