Human Decorin/Bone proteoglycan II (DCN) ELISA Kit

Mfr.No.

CSB-E16522h

Alternative Names

decorin, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, bone proteoglycan II, decorin proteoglycan, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B

Reactivity

Human

Detection Range

3.9 pg/mL-250 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	87
	Range %	82-91
1:4	Average %	94
	Range %	90-99
1:8	Average %	85
	Range %	80-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.496	2.362	2.429	2.282
200	1.951	1.841	1.896	1.749
100	1.349	1.319	1.334	1.187
50	0.935	0.885	0.910	0.763
25	0.603	0.627	0.615	0.468
12.5	0.367	0.374	0.371	0.224
6.25	0.228	0.236	0.232	0.085
0	0.144	0.150	0.147	?

Species

Homo sapiens

Sensitivity

0.97 pg/mL

Limit Of Detection

450 nm

UniProt ID	P07585
Antigen	Decorin/Bone proteoglycan II (DCN)

Applications

ELISA

Research Area

DCN

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502082	CSB-E16522h	96T	$695	Add to Cart
BHE10502082	CSB-E16522h	5x96T	$3336	Add to Cart
BHE10502082	CSB-E16522h	10x96T	$6602.50	Add to Cart

Human Decorin/Bone proteoglycan II (DCN) ELISA Kit

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