Human death-associated protein kinase (DAPK) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14181h

Alternative Names

death-associated protein kinase 1, DAPK, DKFZp781I035

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	94-99
EDTA plasma (n=4)	91	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	109
	Range %	104-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	92
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	P53355
Antigen	death-associated protein kinase 1

Applications

ELISA

Research Area

DAPK1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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