Matrices listed below were spiked with certain level of DDX1 and the recovery rates were calculated by comparing the measured value to the expected amount of DDX1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
91
EDTA plasma(n=5)
86-93
90
heparin plasma(n=5)
96-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DDX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
87-105%
88-105%
EDTA plasma(n=5)
88-100%
85-100%
86-100%
heparin plasma(n=5)
85-93%
85-94%
86-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
DDX1 ELISA Kit allows for the in vitro quantitative determination of DDX1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Q92499
Reviews of Human DDX1(ATP-dependent RNA helicase DDX1) ELISA Kit