Matrices listed below were spiked with certain level of D2D and the recovery rates were calculated by comparing the measured value to the expected amount of D2D in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
93
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
87-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D2D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
D2D ELISA Kit allows for the in vitro quantitative determination of D2D concentrations in serum, plasma, tissue homogenates and other biological fluids.