Human D-Dimer,D2D ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E05175h

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

D-Dimer,D2D

Recovery

The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	90-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	96
	Range %	92-104
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	D2D

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune activation throughout a boosted darunavir monotherapy simplification strategy Benmarzouk-Hidalgo OJ et al,Clin Microbiol Infect,2013 Dec 26. Increased fibrinogen, d-dimer and galectin-3 levels in patients with migraine Yucel Y et al,Neurol Sci,2013 Sep 22. D-Dimer, A ThrombogenicMarker for Diagnosis of IHD in Menopausal Women AN AL-DEJALY et al,International Journal of Scientific Engineering and Technology Research,2014 Sep; 3(28):5608-5610

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