Human D-3-Phosphoglycerate Dehydrogenase

Mfr.No.

E2120Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

2-oxoglutarate reductase;3PGDH;3-PGDH;D-3-phosphoglycerate dehydrogenase;HEL-S-113;Malate dehydrogenase;NLS;NLS1;PDG;PGAD;PGD;PGDH;PHGDH;PHGDHD

Molecular Weight

56651

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2120Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.14	0.2	3.98
Intra-Assay	2	18	1.15	0.07	5.98
Intra-Assay	3	18	4.45	0.3	6.75

Background

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

Protocol

These standard curves of E2120Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.03	2.159	2.089
	2.288
6ng/ml	1.462	1.377	1.308
	1.292
3ng/ml	0.925	0.94	0.871
	0.955
1.5ng/ml	0.475	0.499	0.43
	0.523
0.75ng/ml	0.22	0.244	0.175
	0.269
0ng/ml	0.07	0.069	0
	0.068

Gene ID	26227
UniProt ID	O43175

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103664	E2120Hu	48T	$320		Add to Cart
BHE12103664	E2120Hu	96T	$458		Add to Cart

Human D-3-Phosphoglycerate Dehydrogenase - PHGDH ELISA Kit

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