Human Cytotoxic T Lymphocyte Associated Antigen 4

Mfr.No.

E0277Hu

Sensitivity

0.25ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Cytotoxic T Lymphocyte Associated Antigen 4 (also known as CTLA-4) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD152;CTLA 4;CTLA4;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Molecular Weight

24656

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0277Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	44.8	1.37	3
Intra-Assay	2	18	31.4	1.61	5.1
Intra-Assay	3	18	9.4	0.67	7.2

Background

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Protocol

These standard curves of E0277Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.071	2.232	2.189
	2.393
40ng/ml	1.137	1.248	1.205
	1.36
20ng/ml	0.797	0.757	0.714
	0.717
10ng/ml	0.52	0.456	0.413
	0.392
5ng/ml	0.179	0.192	0.149
	0.206
0ng/ml	0.033	0.043	0
	0.054

Gene ID	1493
UniProt ID	P16410

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101990	E0277Hu	96T	$458		Add to Cart
BHE12101990	E0277Hu	48T	$320		Add to Cart

Human Cytotoxic T Lymphocyte Associated Antigen 4 - CTLA-4 ELISA Kit

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