Human Cytoplasmic Dynein 1 Heavy Chain 1

Mfr.No.

E5166Hu

Alternative Names

Cytoplasmic dynein 1 heavy chain 1;Cytoplasmic dynein heavy chain 1;DYNC 1H1;DYNC1H1;Dynein heavy chain, cytosolic

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

532408

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.66ng/L

Background

Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression (PubMed: 27462074).

Gene ID	1778
UniProt ID	Q14204
Antigen	DYNC1H1

Research Area

Others

Protocol

These standard curves of E5166Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.299	2.303	2.275
	2.307
600ng/L	1.807	1.769	1.741
	1.731
300ng/L	1.12	1.142	1.114
	1.165
150ng/L	0.682	0.676	0.648
	0.67
75ng/L	0.398	0.419	0.39
	0.439
0ng/L	0.029	0.028	0
	0.028

Precision

We measured random samples of E5166Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	80.7	3.4	4.2
Intra-Assay	2	18	583.2	34.65	5.9
Intra-Assay	3	18	165	10.18	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106529	E5166Hu	48T	$320		Add to Cart
BHE12106529	E5166Hu	96T	$458		Add to Cart

Human Cytoplasmic Dynein 1 Heavy Chain 1 - DYNC1H1 ELISA Kit

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