Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09606h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cytokeratin fragment antigen 21-1,CYFRA21-1

Recovery

The recovery of human NOX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	85-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NOX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	84
	Range %	80-88
1:10	Average %	99
	Range %	95-103
1:20	Average %	88
	Range %	84-92
1:40	Average %	107
	Range %	100-115

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	CYFRA21-1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis Joy Cuenco.et al,Oncotarget,April

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