Human cytokeratin 18,CK-18 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09172h

Alternative Names

keratin 18, CYK18, K18, cell proliferation-inducing protein 46, cytokeratin 18

Reactivity

Human

Detection Range

0.625 ng/mL-15 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human BLC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-105
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BLC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-99
1:2	Average %	96
	Range %	91-100
1:4	Average %	90
	Range %	85-96
1:8	Average %	92
	Range %	83-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.25 ng/mL

Limit Of Detection

450 nm

UniProt ID	P05783
Antigen	cytokein 18,CK-18

Applications

ELISA

Research Area

KRT18

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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