Human Cytohesin-2 - CYTH2 ELISA Kit

Mfr.No.

E5162Hu

Sensitivity

3.04ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ARF exchange factor;ARF nucleotide-binding site opener;CYTH 2;CYTH2;Cytohesin-2;PH, SEC7 and coiled-coil domain-containing protein 2;Protein ARNO

Molecular Weight

46546

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5162Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	104.4	3.46	3.3
Intra-Assay	2	18	250.9	8.82	3.5
Intra-Assay	3	18	418.6	21.25	5.1

Background

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (PubMed: 17398095). Involved in neurite growth (By similarity).

Protocol

These standard curves of E5162Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.081	2.041	2.013
	2.001
320ng/L	1.47	1.516	1.488
	1.561
160ng/L	0.679	0.622	0.594
	0.566
80ng/L	0.377	0.359	0.331
	0.342
40ng/L	0.195	0.191	0.163
	0.188
0ng/L	0.02	0.028	0
	0.036

Gene ID	9266
UniProt ID	Q99418

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106525	E5162Hu	48T	$320		Add to Cart
BHE12106525	E5162Hu	96T	$458		Add to Cart

Human Cytohesin-2 - CYTH2 ELISA Kit

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