Human Cytochrome P450 2E1(CYP2E1) ELISA kit

Mfr.No.

CSB-EL006425HU

Alternative Names

cytochrome P450, family 2, subfamily E, polypeptide 1, CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenas

Reactivity

Human

Detection Range

43.75 pg/mL-2800 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-97
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-102
1:2	Average %	107
	Range %	98-111
1:4	Average %	92
	Range %	84-96
1:8	Average %	99
	Range %	93-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.045	2.105	2.075	1.944
10	1.743	1.794	1.769	1.638
5	1.167	1.199	1.183	1.052
2.5	0.608	0.627	0.618	0.487
1.25	0.309	0.314	0.312	0.181
0.625	0.201	0.213	0.207	0.076
0.312	0.165	0.161	0.163	0.032
0	0.133	0.129	0.131	?

Species

Homo sapiens

Sensitivity

10.94 pg/mL

Limit Of Detection

450 nm

UniProt ID	P05181
Antigen	cytochrome P450, family 2, subfamily E, polypeptide 1

Applications

ELISA

Research Area

CYP2E1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502045	CSB-EL006425HU	96T	$695	Add to Cart
BHE10502045	CSB-EL006425HU	5x96T	$3336	Add to Cart
BHE10502045	CSB-EL006425HU	10x96T	$6602.50	Add to Cart

Human Cytochrome P450 2E1(CYP2E1) ELISA kit

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