Human Cytochrome P450 2C9(CYP2C9) ELISA kit

Human Cytochrome P450 2C9(CYP2C9) ELISA kit

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Mfr.No.

CSB-EL006419HU

Alternative Names

cytochrome P450, family 2, subfamily C, polypeptide 9, CPC9, CYP2C, CYP2C10, MGC149605, MGC88320, P450IIC9, cytochrome P-450 S-mephenytoin 4-hydroxylase, cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 10, cytochrome P450, subfamil

Reactivity

Human

Detection Range

23.5 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	89
	Range %	85-98
1:8	Average %	101
	Range %	95-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.932	2.789	2.861	2.771
1000	2.178	2.132	2.155	2.065
500	1.566	1.555	1.561	1.471
250	0.789	0.778	0.784	0.694
125	0.462	0.456	0.459	0.369
62.5	0.278	0.267	0.273	0.183
31.25	0.226	0.218	0.222	0.132
0	0.093	0.087	0.090	?

Species

Homo sapiens

Sensitivity

5.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P11712
Antigen	cytochrome P450, family 2, subfamily C, polypeptide 9

Applications

ELISA

Research Area

CYP2C9

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502044	CSB-EL006419HU	96T	$695	Add to Cart
BHE10502044	CSB-EL006419HU	5x96T	$3336	Add to Cart
BHE10502044	CSB-EL006419HU	10x96T	$6602.50	Add to Cart

The recovery of human ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)