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Home / ELISA Kit

Human Cytochrome B5 - CYB5A ELISA Kit

SKU : BHE12106516

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

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Bioassay Technology Laboratory

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Mfr.No.

E5153Hu

Sensitivity

7.02ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CYB5;CYB5A;Cytochrome b5;MCB5;METAG;Microsomal cytochrome b5 type A

Molecular Weight

15330

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5153Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1121.29	27.69	2.47
Intra-Assay	2	18	316.94	12.41	3.92
Intra-Assay	3	18	980.8	45.37	4.63

Background

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

Protocol

These standard curves of E5153Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.925	2.002	1.941
	2.079
800ng/L	0.95	0.962	0.902
	0.975
400ng/L	0.489	0.531	0.47
	0.573
200ng/L	0.27	0.245	0.184
	0.22
100ng/L	0.085	0.081	0.02
	0.077
0ng/L	0.059	0.061	0
	0.062

Gene ID	1528
UniProt ID	P00167

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106516	E5153Hu	48T	$320		Add to Cart
BHE12106516	E5153Hu	96T	$458		Add to Cart

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