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Home / ELISA Kit

Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit

SKU : BHE13702303

UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR,Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site). Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.

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Mfr.No.	KTE60084
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCR.
Alternative Names	UQCR; 0710008D09Rik; QCR10; UQCR11; complexI subunit XI; cytochrome b-c1 complex subunit 10; ubiquinol cytochrome c reductase; 6.4kDa subunit
Detection Method	Colorimetric
Kit Components	Human Cytochrome b-c1 complex subunit 10 microplate Human Cytochrome b-c1 complex subunit 10 standard Human Cytochrome b-c1 complex subunit 10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit has high sensitivity and excellent specificity for detection of Human UQCR. No significant cross-reactivity or interference between Human UQCR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR,Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site). Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.
Notes	This Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCR in samples. An antibody specific for UQCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10975

Applications	ELISA
Applications Notes	This Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCR in samples. An antibody specific for UQCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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