Human Cytochrome b-245 Beta Polypeptide ELISA Kit

Mfr.No.

EH4124

Alternative Names

NOX2 ELISA Kit, CGD91-phox ELISA Kit, NADPH oxidase 2 ELISA Kit, gp91phox ELISA Kit, p22 phagocyte B-cytochrome ELISA Kit, gp91-1 ELISA Kit, Superoxide-geneRating NADPH oxidase heavy chain subunit ELISA Kit, Neutrophil cytochrome b 91 kDa polypeptide ELISA Kit, Heme-binding membrane glycoprotein gp91phox ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of CYBB and the recovery rates were calculated by comparing the measured value to the expected amount of CYBB in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-97	90
EDTA plasma(n=5)	90-105	99
heparin plasma(n=5)	88-104	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYBB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	95-105%	92-105%	86-99%
EDTA plasma(n=5)	86-99%	82-94%	82-100%
heparin plasma(n=5)	80-100%	87-96%	85-92%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

NOX2/CGD91-phox/NADPH oxidase 2/gp91phox/p22 phagocyte B-cytochrome/gp91-1/Superoxide-generating NADPH oxidase heavy chain subunit/Neutrophil cytochrome b 91 kDa polypeptide/Heme-binding membrane glycoprotein gp91phox

Notes

For Research Use Only

UniProt ID	P04839
Antigen	CYBB

Applications	ELISA
Applications Notes	Cytochrome b-245 Beta Polypeptide ELISA Kit allows for the in vitro quantitative determination of CYBB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800535	EH4124	96T	$400		Add to Cart

Human Cytochrome b-245 Beta Polypeptide ELISA Kit

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