Human Cystatin-Sn - CST1 ELISA Kit

Mfr.No.

E2801Hu

Sensitivity

0.023ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CST 1;CST1;Cystain-SA-I;Cystatin-1;Cystatin-SN;Salivary cystatin-SA-1

Molecular Weight

16388

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2801Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.1	0.13	4.2
Intra-Assay	2	18	6.3	0.17	2.7
Intra-Assay	3	18	1.5	0.09	5.9

Background

Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.

Protocol

These standard curves of E2801Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.322	2.384	2.313
	2.445
6ng/ml	1.238	1.332	1.262
	1.427
3ng/ml	0.695	0.731	0.661
	0.768
1.5ng/ml	0.443	0.453	0.383
	0.463
0.75ng/ml	0.328	0.295	0.225
	0.262
0ng/ml	0.064	0.07	0
	0.076

Gene ID	1469
UniProt ID	P01037

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104338	E2801Hu	48T	$320		Add to Cart
BHE12104338	E2801Hu	96T	$458		Add to Cart

Human Cystatin-Sn - CST1 ELISA Kit

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