Human cystathionine beta-synthase(CBS) ELISA Kit

Mfr.No.

CSB-E13314h

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cystathionine-beta-synthase, HIP4, OTTHUMP00000109415, OTTHUMP00000109416, OTTHUMP00000109418, beta-thionase, cystathionine beta-synthase, methylcysteine synthase, serine sulfhydrase, cystathionine beta-synthase(CBS), cystathionine beta-synthase(CBS)

Recovery

The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	95	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-105
1:2	Average %	89
	Range %	83-96
1:4	Average %	92
	Range %	84-99
1:8	Average %	91
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.321	0.332	0.327
12.5	0.512	0.537	0.525
3.12	0.826	0.851	0.839
0.78	1.175	1.153	1.164
0.195	1.655	1.632	1.644
0	1.985	1.948	1.967

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P35520

Applications	ELISA
Research Area	CBS

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502013	CSB-E13314h	96T	$790	Add to Cart
BHE10502013	CSB-E13314h	5x96T	$3792	Add to Cart
BHE10502013	CSB-E13314h	10x96T	$7505	Add to Cart

Human cystathionine beta-synthase(CBS) ELISA Kit

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