Human CYP7A1(Cholesterol 7-alpha-monooxygenase) ELISA Kit

Mfr.No.

EH1191

Alternative Names

CYP7A1 ELISA Kit, Cytochrome P450 7A1 ELISA Kit, Cholesterol 7-alpha-monooxygenase ELISA Kit, CYPVII ELISA Kit, Cholesterol 7-alpha-hydroxylase ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of CYP7A1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP7A1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	90-99	94
EDTA plasma(n=5)	95-103	98
heparin plasma(n=5)	85-97	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP7A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-104%	90-99%	87-91%
EDTA plasma(n=5)	82-94%	85-97%	83-95%
heparin plasma(n=5)	82-100%	81-93%	84-94%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

CYP7A1(Cytochrome P450 7A1)/Cholesterol 7-alpha-monooxygenase/CYPVII/Cholesterol 7-alpha-hydroxylase

Notes

For Research Use Only

UniProt ID	P22680
Antigen	CYP7A1

Applications	ELISA
Applications Notes	CYP7A1 ELISA Kit allows for the in vitro quantitative determination of CYP7A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806962	EH1191	96T	$400		Add to Cart

Human CYP7A1(Cholesterol 7-alpha-monooxygenase) ELISA Kit

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