Human cyclophilin B,CyPB ELISA Kit

Human cyclophilin B,CyPB ELISA Kit

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Mfr.No.

CSB-E11218h

Alternative Names

peptidylprolyl isomerase B (cyclophilin B), CYP-S1, CYPB, MGC14109, MGC2224, OI9, SCYLP, PPIase, S-cyclophilin, cyclophilin-like protein, peptidyl-prolyl cis-trans isomerase B, peptidylprolyl isomerase B, rotamase

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit IL-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	82-104
EDTA plasma (n=4)	95	85-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	85-105
1:2	Average %	95
	Range %	85-103
1:4	Average %	94
	Range %	82-100
1:8	Average %	95
	Range %	85-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.852	2.713	2.783	2.674
250	2.235	2.245	2.240	2.131
125	1.424	1.392	1.408	1.299
62.5	0.753	0.784	0.769	0.660
31.2	0.447	0.433	0.440	0.331
15.6	0.266	0.282	0.274	0.165
7.8	0.202	0.195	0.199	0.090
0	0.111	0.106	0.109	?

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P23284
Antigen	peptidylprolyl isomerase B (cyclophilin B)

Applications

ELISA

Research Area

PPIB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502011	CSB-E11218h	96T	$695	Add to Cart
BHE10502011	CSB-E11218h	5x96T	$3336	Add to Cart
BHE10502011	CSB-E11218h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.