Human cyclooxygenase-2,COX-2 ELISA Kit

Human cyclooxygenase-2,COX-2 ELISA Kit

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Mfr.No.

CSB-E10103h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, cyclooxygenase 2b, prostaglandin G/H synthase and cyclooxygenase, prostaglandin-endoperoxide synthase 2

Recovery

The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-108
EDTA plasma (n=4)	91	86-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-96
1:2	Average %	96
	Range %	91-104
1:4	Average %	97
	Range %	92-105
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.052	2.011	2.032	1.900
15	1.384	1.283	1.334	1.202
7.5	0.868	0.847	0.858	0.726
3.75	0.501	0.512	0.507	0.375
1.8	0.326	0.337	0.332	0.200
0.9	0.243	0.254	0.249	0.117
0.45	0.198	0.194	0.196	0.064
0	0.132	0.131	0.132	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35354

Applications	ELISA
Research Area	PTGS2

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.