Human cyclooxygenase-2,COX-2 ELISA Kit

Human cyclooxygenase-2,COX-2 ELISA Kit

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Mfr.No.

CSB-E10103h

Alternative Names

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, cyclooxygenase 2b, prostaglandin G/H synthase and cyclooxygenase, prostaglandin-endoperoxide synthase 2

Reactivity

Human

Detection Range

1.25 ng/mL-80 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-108
EDTA plasma (n=4)	91	86-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-96
1:2	Average %	96
	Range %	91-104
1:4	Average %	97
	Range %	92-105
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.052	2.011	2.032	1.900
15	1.384	1.283	1.334	1.202
7.5	0.868	0.847	0.858	0.726
3.75	0.501	0.512	0.507	0.375
1.8	0.326	0.337	0.332	0.200
0.9	0.243	0.254	0.249	0.117
0.45	0.198	0.194	0.196	0.064
0	0.132	0.131	0.132	?

Species

Homo sapiens

Sensitivity

0.31 ng/mL

Limit Of Detection

450 nm

UniProt ID	P35354
Antigen	prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

Applications

ELISA

Research Area

PTGS2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502009	CSB-E10103h	96T	$790	Add to Cart
BHE10502009	CSB-E10103h	5x96T	$3792	Add to Cart
BHE10502009	CSB-E10103h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.