Human Cyclin-Y - CCNY ELISA Kit

Mfr.No.

E5142Hu

Sensitivity

4.39ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C10orf9;CBCP1;CCNX;CCNY;CFP1;Cyclin box protein 1;Cyclin fold protein 1;cyclin-X;Cyclin-Y;Cyc-Y

Molecular Weight

39337

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5142Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	93.97	4.66	4.96
Intra-Assay	2	18	247.25	13.26	5.36
Intra-Assay	3	18	325.06	12.33	3.79

Background

Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.

Protocol

These standard curves of E5142Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.135	2.158	2.108
	2.18
600ng/L	1.731	1.823	1.773
	1.914
300ng/L	1.187	1.204	1.154
	1.221
150ng/L	0.6	0.627	0.577
	0.655
75ng/L	0.348	0.355	0.305
	0.362
0ng/L	0.038	0.05	0
	0.062

Gene ID	219771
UniProt ID	Q8ND76

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106505	E5142Hu	48T	$320		Add to Cart
BHE12106505	E5142Hu	96T	$458		Add to Cart

Human Cyclin-Y - CCNY ELISA Kit

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