Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3(CDKN2A) ELISA kit

Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3(CDKN2A) ELISA kit

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Mfr.No.

CSB-EL005089HU

Alternative Names

cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19, p19Arf, CDK4 inhibitor p16-INK4, OTTHUMP00000021148, cell cycle negative regulator beta, cyclin-dependen

Reactivity

Human

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human OGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-104
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	96
	Range %	91-105
1:10	Average %	95
	Range %	92-98
1:20	Average %	87
	Range %	80-92
1:40	Average %	99
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.432	2.332	2.382	2.254
5	1.804	1.704	1.754	1.626
2.5	1.187	1.287	1.237	1.109
1.25	0.716	0.726	0.721	0.593
0.625	0.447	0.457	0.452	0.324
0.312	0.345	0.355	0.350	0.222
0.156	0.227	0.237	0.232	0.104
0	0.129	0.126	0.128	?

Species

Homo sapiens

Sensitivity

4.69 pg/mL

Limit Of Detection

450 nm

UniProt ID	P42771
Antigen	cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Applications

ELISA

Research Area

CDKN2A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502007	CSB-EL005089HU	96T	$695	Add to Cart
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BHE10502007	CSB-EL005089HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.