Human Cyclin-dependent Kinase Inhibitor 2A

Mfr.No.

E5141Hu

Sensitivity

1.73ng/ml

Detection Range

3.75-240ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ARF;CDK4I;CDKN2;CDKN2A;CMM2;Cyclin-dependent kinase 4 inhibitor A;Cyclin-dependent kinase inhibitor 2A;INK4A;MLM;MTS1;MTS-1;Multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;p16-INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16;

Molecular Weight

16533

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5141Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	63.9	3.2	5.1
Intra-Assay	2	18	180.9	8.1	4.5
Intra-Assay	3	18	81.1	4.2	5.2

Background

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Protocol

These standard curves of E5141Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	1.979	1.93	1.892
	1.882
120ng/ml	1.312	1.263	1.225
	1.215
60ng/ml	0.681	0.669	0.63
	0.656
30ng/ml	0.346	0.329	0.291
	0.312
15ng/ml	0.187	0.175	0.137
	0.164
0ng/ml	0.036	0.038	0
	0.04

Gene ID	1029
UniProt ID	P42771

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106504	E5141Hu	96T	$458		Add to Cart
BHE12106504	E5141Hu	48T	$320		Add to Cart

Human Cyclin-dependent Kinase Inhibitor 2A - CDKN2A ELISA Kit

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