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Home / ELISA Kit

Human Cyclin-dependent Kinase 4 Inhibitor B - CDKN2B ELISA Kit

SKU : BHE12106503

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

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Bioassay Technology Laboratory

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+1 866.986.9598

Email

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Mfr.No.

E5140Hu

Sensitivity

9.42ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CDKN2B;Cyclin-dependent kinase 4 inhibitor B;MTS-2;Multiple tumor suppressor 2;p14-INK4b;p15INK4B;p15-INK4b

Molecular Weight

14722

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5140Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	623	24.83	4
Intra-Assay	2	18	1542.8	49.32	3.2
Intra-Assay	3	18	1045.7	50.36	4.8

Background

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

Protocol

These standard curves of E5140Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.312	2.267	2.236
	2.221
1200ng/L	1.359	1.344	1.314
	1.33
600ng/L	0.614	0.645	0.615
	0.677
300ng/L	0.361	0.364	0.333
	0.367
150ng/L	0.121	0.128	0.098
	0.135
0ng/L	0.041	0.031	0
	0.02

Gene ID	1030
UniProt ID	P42772

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106503	E5140Hu	48T	$320		Add to Cart
BHE12106503	E5140Hu	96T	$458		Add to Cart

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