Human CXCL9/Mig ELISA Kit PicoKine®

Mfr.No.

EK0732

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.066	0.165	0.227	0.367	0.624	1.030	1.341	2.040

Description

Human CXCL9/Mig ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL9 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 9;Gamma-interferon-induced monokine;Monokine induced by interferon-gamma;HuMIG;MIG;Small-inducible cytokine B9;CXCL9;CMK, MIG, SCYB9;

Molecular Weight

14019 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T23-T125

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CXCL9

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	84	277	773	77	272	823
Standard deviation	6.55	11.63	57.2	6.16	11.69	60.9
CV (%)	7.8%	4.2%	7.4%	8%	4.3%	7.4%

Protein Function

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Background

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	84	94	97	93	92	4.84	5.2%
Sample 2	277	262	265	250	263	9.6	3.6%
Sample 3	773	838	880	794	821	41.23	5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4283
Gene Name	CXCL9
UniProt ID	Q07325
Protein Name	C-X-C motif chemokine 9
Sequence	Belongs to the intercrine alpha (chemokine CxC) family.

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000592	EK0732	96 wells/kit	$499		Add to Cart

Human CXCL9/Mig ELISA Kit PicoKine®

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