Human CXCL1/Gro Alpha ELISA Kit PicoKine®

Mfr.No.

EK0722

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.044	0.098	0.203	0.309	0.498	0.877	1.335	1.967

Description

Human CXCL1/Gro Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth-regulated alpha protein;C-X-C motif chemokine 1;GRO-alpha (1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3;GRO-alpha (4-73);GRO-alpha (5-73);GRO-alpha (6-73);CXCL1;GRO, GRO1, GROA, MGSA, SCYB1;

Molecular Weight

11301 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A35-N107

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CXCL1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	194	451	36	204	438
Standard deviation	2.44	9.7	29.31	2.95	10.81	36.51
CV (%)	7.2%	5%	6.5%	8.2%	5.3%	8.2%

Protein Function

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha (4-73), GRO-alpha (5-73) and GRO-alpha (6-73) show a 30-fold higher chemotactic activity. .

Background

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	35	39	33	35	2.27	6.4%
Sample 2	194	234	212	219	214	14.37	6.7%
Sample 3	451	412	454	480	449	24.28	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2919
Gene Name	CXCL1
UniProt ID	P09341
Protein Name	Growth-regulated alpha protein
Sequence	Belongs to the intercrine alpha (chemokine CxC) family.

Applications	ELISA
Research Area	Cytokines,G Protein Signaling,Immunology,Innate Immunity,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000586	EK0722	96 wells/kit	$499		Add to Cart

Human CXCL1/Gro Alpha ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CXCL1/Gro Alpha ELISA Kit PicoKine®

Rate it now..!!