Human CXC-chemokine receptor 4(CXCR4)ELISA Kit

Human CXC-chemokine receptor 4(CXCR4)ELISA Kit

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Mfr.No.

CSB-E12825h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-X-C motif) receptor 4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, C-X-C chemokine receptor type 4, CD184 antigen, fusin, leukocyte-derived seven-transmembrane-domain receptor, lip

Recovery

The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	106	100-114

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	85
	Range %	80-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.607	2.497	2.552	2.378
50	2.203	2.075	2.139	1.965
25	1.635	1.542	1.589	1.415
12.5	0.952	1.006	0.979	0.805
6.25	0.610	0.587	0.599	0.425
3.12	0.451	0.462	0.457	0.283
1.56	0.332	0.315	0.324	0.150
0	0.176	0.171	0.174

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P61073

Applications	ELISA
Research Area	CXCR4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501996	CSB-E12825h	96T	$695	Add to Cart
BHE10501996	CSB-E12825h	5x96T	$3336	Add to Cart
BHE10501996	CSB-E12825h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.