Human Cx3C-Chemokine Receptor 1

Mfr.No.

E0296Hu

Description

This sandwich kit is for the accurate quantitative detection of human X3C-chemokine Receptor 1 (also known as CX3CR1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Beta chemokine receptor-like 1;CMK-BRL1;CMK-BRL-1;CX3C chemokine receptor 1;C-X3-C CKR-1;CX3CR1;Fractalkine receptor;G-protein coupled receptor 13;V28;

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

40396

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.47ng/L

Background

Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions (PubMed: 9390561, PubMed: 23125415). Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro) (PubMed: 9726990). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1 (PubMed: 14607932).

Gene ID	1524
UniProt ID	P49238
Antigen	CX3CR1

Research Area

Immunology

Protocol

These standard curves of E0296Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.202	2.198	2.137
	2.193
1200ng/L	1.36	1.297	1.237
	1.233
600ng/L	0.878	0.85	0.79
	0.823
300ng/L	0.526	0.513	0.453
	0.5
150ng/L	0.238	0.245	0.185
	0.252
0ng/L	0.042	0.06	0
	0.079

Precision

We measured random samples of E0296Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	345.2	19.04	5.5
Intra-Assay	2	18	1912.1	52.17	2.7
Intra-Assay	3	18	875.9	40.81	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102003	E0296Hu	96T	$458		Add to Cart
BHE12102003	E0296Hu	48T	$320		Add to Cart

Human Cx3C-Chemokine Receptor 1 - CX3CR1 ELISA Kit

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