Matrices listed below were spiked with certain level of CTSF and the recovery rates were calculated by comparing the measured value to the expected amount of CTSF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
99
EDTA plasma(n=5)
88-93
90
heparin plasma(n=5)
87-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CTSF ELISA Kit allows for the in vitro quantitative determination of CTSF concentrations in serum, plasma, tissue homogenates and other biological fluids.