Matrices listed below were spiked with certain level of CS and the recovery rates were calculated by comparing the measured value to the expected amount of CS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
92
EDTA plasma(n=5)
90-102
95
heparin plasma(n=5)
94-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.