Matrices listed below were spiked with certain level of CRTC2/TORC2 and the recovery rates were calculated by comparing the measured value to the expected amount of CRTC2/TORC2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
99
EDTA plasma(n=5)
87-101
95
heparin plasma(n=5)
85-98
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRTC2/TORC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-93%
90-98%
85-102%
EDTA plasma(n=5)
89-99%
88-100%
84-100%
heparin plasma(n=5)
83-99%
82-95%
81-94%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q53ET0
Antigen
CRTC2
Applications
ELISA
Applications Notes
CRTC2 ELISA Kit allows for the in vitro quantitative determination of CRTC2/TORC2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
4 °C for 6 months
Reviews of Human CRTC2(CREB-regulated transcription coactivator 2) ELISA Kit