Matrices listed below were spiked with certain level of CRTAC1 and the recovery rates were calculated by comparing the measured value to the expected amount of CRTAC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-95
91
EDTA plasma(n=5)
92-102
96
heparin plasma(n=5)
87-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRTAC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-103%
93-99%
87-103%
EDTA plasma(n=5)
89-99%
89-96%
92-100%
heparin plasma(n=5)
86-95%
87-92%
87-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9NQ79
Applications
ELISA
Applications Notes
CRTAC1 ELISA Kit allows for the in vitro quantitative determination of CRTAC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CRTAC1(Cartilage acidic protein 1) ELISA Kit