Human CRP/C Reactive Protein ELISA Kit PicoKine® (96 Tests). Quantitate Human CRP in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Alternative Names
C-reactive protein;C-reactive protein (1-205);CRP;PTX1;
Molecular Weight
25039 MW
Immunogen
Expression system for standard: NS0; Immunogen sequence: F17-P224
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Background
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
Reproducibility
Lots
Lot 1 (pg/ml)
Lot 2 (pg/ml)
Lot 3 (pg/ml)
Lot 4 (pg/ml)
Mean (pg/ml)
Standard Deviation
CV (%)
Sample 1
593
514
598
593
574
34.98
6%
Sample 2
1488
1753
1588
1771
1650
117.62
7.1%
Sample 3
8142
7585
8200
7898
7956
242.45
3%
*number of samples for each test n=16.
Notes
Material Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer . Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Gene ID
1401
Gene Name
CRP
UniProt ID
P02741
Protein Name
C-reactive protein
Tissue Specificity
Found in plasma.
Sequence
Belongs to the pentaxin family.
Applications
ELISA
Research Area
Epigenetics and Nuclear Signaling,Metabolism,Immunology,Apoptosis,Atherosclerosis,Cardiovascular,Chromatin Binding Proteins,DNA/RNA Binding,Innate Immunity,Macrophage/Inflammation,Vascular Inflammation,Complement
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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