Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit

Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit

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Mfr.No.

CSB-E11224h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I, Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I, Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I

Recovery

The recovery of mouse PPAR-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	107	103-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPAR-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	94
	Range %	90-98
1:2000	Average %	84
	Range %	80-88
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	83
	Range %	80-86

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
40	2.686	2.598	2.642	2.550
20	2.207	2.241	2.224	2.132
10	1.503	1.562	1.533	1.441
5	0.898	0.886	0.892	0.800
2.5	0.423	0.438	0.431	0.339
1.25	0.304	0.326	0.315	0.223
0.625	0.201	0.215	0.208	0.116
0	0.091	0.093	0.092

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	CTX-I

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Alteration of Cartilage Degeneration and InflammationMarkers in Temporomandibular Joint Osteoarthritis Occurs Proportionally Vos LM et al,J Oral Maxillofac Surg,2013 Aug 8.pii: S0278-2391(13)00806-9. Combination Treatment with Whole Body Vibration and a Kidney-tonifying Herbal Fufang Prevent Osteoporosis in Ovariectomized Rats Wei QS et al,Orthop Surg,2017 Feb;7(1):57-65. Effect of Vitamin D on Peripheral Blood Mononuclear Cells from Patients with Psoriasis Vulgaris and Psoriatic Arthritis Cubillos S.et al,PLoS One.,6;11(4 RANKL/Osteoprotegerin System and Bone Turnover in Hashimoto Thyroiditis. Konca Degertekin C.et al,Calcif Tissue Int.,Jan 8 The Circadian Gene Clock Regulates Bone Formation Via PDIA3 Yuan G.et al,J Bone Miner Res. ,Nov 24.

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SKU	Supplier No.	Size	Your price
BHE10501969	CSB-E11224h	96T	$695	Add to Cart
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BHE10501969	CSB-E11224h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.