Human CRLF2/TSLP R ELISA Kit PicoKine®

Mfr.No.

EK1288

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.034	0.090	0.146	0.249	0.497	0.931	1.489	2.095

Description

Human CRLF2/TSLP R ELISA Kit PicoKine® (96 Tests). Quantitate Human CRLF2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cytokine receptor-like factor 2;Cytokine receptor-like 2;IL-XR;Thymic stromal lymphopoietin protein receptor;TSLP receptor;CRLF2;CRL2, ILXR, TSLPR;

Molecular Weight

42013 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G25-K231

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CRLF2

Subcellular Localization

Isoform 1: Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	110	661	1213	107	721	1176
Standard deviation	5.17	27.1	73.99	5.24	37.49	88.2
CV (%)	4.7%	4.1%	6.1%	4.9%	5.2%	7.5%

Protein Function

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. .

Background

Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	110	110	114	115	112	2.27	2%
Sample 2	661	633	642	685	655	19.92	3%
Sample 3	1213	1047	1166	1231	1164	71.73	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	64109
Gene Name	CRLF2
UniProt ID	Q9HC73
Protein Name	Cytokine receptor-like factor 2
Tissue Specificity	Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.
Sequence	Belongs to the type I cytokine receptor family. Type 5 subfamily.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000877	EK1288	96 wells/kit	$750		Add to Cart

Human CRLF2/TSLP R ELISA Kit PicoKine®

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