Human Crk-like Protein - CRKL ELISA Kit

Mfr.No.

E5125Hu

Sensitivity

9.54ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CRKL;Crk-like protein

Molecular Weight

33777

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5125Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	798.39	45.32	5.68
Intra-Assay	2	18	1924.49	64.01	3.33
Intra-Assay	3	18	539.49	34.1	6.32

Background

Crk-like protein is a protein that in humans is encoded by the CRKL gene. v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.

Protocol

These standard curves of E5125Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.146	2.166	2.107
	2.187
1200ng/L	1.149	1.25	1.19
	1.35
600ng/L	0.682	0.737	0.678
	0.793
300ng/L	0.532	0.501	0.441
	0.469
150ng/L	0.173	0.192	0.132
	0.211
0ng/L	0.058	0.06	0
	0.061

Gene ID	1399
UniProt ID	P46109

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106488	E5125Hu	48T	$320		Add to Cart
BHE12106488	E5125Hu	96T	$458		Add to Cart

Human Crk-like Protein - CRKL ELISA Kit

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