Matrices listed below were spiked with certain level of CRH and the recovery rates were calculated by comparing the measured value to the expected amount of CRH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
94
EDTA plasma(n=5)
89-99
95
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CRH ELISA Kit allows for the in vitro quantitative determination of CRH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CRH(Corticotropin Releasing Hormone) ELISA Kit