Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Mfr.No.

CSB-E05140h

Sensitivity

0.22 ng/mL

Detection Range

0.9 ng/mL-60 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Creatine Kinase MB isoenzyme,CK-MB

Recovery

The recovery of human TRAF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-94
EDTA plasma (n=4)	107	103-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-90
1:2	Average %	101
	Range %	98-106
1:4	Average %	92
	Range %	85-96
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	CK-MB

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Amelioration of ER stress by 4-phenylbutyric acid reduces chronic hypoxia induced cardiac damage and improves hypoxic tolerance through upregulation of HIF-1a Jain K.et al,Vascul Pharmacol.,83:36-46

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