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Home / ELISA Kit

Human Cornifin-A (SPRR1A) ELISA Kit

SKU : BHE13702563

SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo.The most characteristic feature of the SPRR gene family resides in the structure of the central segment of the encoded polypeptides that have built up from tandemly repeated units of either 8 (in SPRR1 and SPRR3) or 9 (in SPRR2) amino acids with the general consensus *K*PEP**. The sequence of the different members of this family of genes together with their clustered chromosomal organization strongly suggests that these genes have evolved from a single progenitor gene by multiple intra- and intergenic duplications. Analysis of the different SPRR subfamilies revealed a gene-specific bias to either intra- and intergenic duplication.

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Mfr.No.	KTE60346
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Cornifin-A (SPRR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR1A.
Alternative Names	SPRR1A; SPRK; OTTHUMP00000016121; cornifin A
Detection Method	Colorimetric
Kit Components	Human Cornifin-A microplate Human Cornifin-A standard Human Cornifin-A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Cornifin-A (SPRR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPRR1A. No significant cross-reactivity or interference between Human SPRR1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo.The most characteristic feature of the SPRR gene family resides in the structure of the central segment of the encoded polypeptides that have built up from tandemly repeated units of either 8 (in SPRR1 and SPRR3) or 9 (in SPRR2) amino acids with the general consensus KPEP**. The sequence of the different members of this family of genes together with their clustered chromosomal organization strongly suggests that these genes have evolved from a single progenitor gene by multiple intra- and intergenic duplications. Analysis of the different SPRR subfamilies revealed a gene-specific bias to either intra- and intergenic duplication.
Notes	This Human Cornifin-A (SPRR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR1A in samples. An antibody specific for SPRR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6698

Applications	ELISA
Applications Notes	This Human Cornifin-A (SPRR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR1A in samples. An antibody specific for SPRR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702563	KTE60346	48 T	$339		Add to Cart
BHE13702563	KTE60346	96 T	$589		Add to Cart
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BHE13702563	KTE60346	96 Tx50	$24569		Add to Cart

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