Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit

Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit

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Mfr.No.

CSB-E12935h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

runt-related transcription factor 2, RP1-166H4.1, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, CBF-alpha 1, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A

Recovery

The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	85-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	105
	Range %	101-109
1:200	Average %	87
	Range %	84-90
1:400	Average %	102
	Range %	98-106
1:800	Average %	105
	Range %	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?U/ml	OD1	OD2	Average
500	0.080	0.078	0.079
250	0.129	0.131	0.130
125	0.201	0.215	0.208
62.5	0.321	0.348	0.335
31.25	0.542	0.501	0.522
15.6	0.838	0.868	0.853
7.8	1.168	1.198	1.183
0	2.025	1.987	2.006

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13950

Applications	ELISA
Research Area	RUNX2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501946	CSB-E12935h	96T	$790	Add to Cart
BHE10501946	CSB-E12935h	5x96T	$3792	Add to Cart
BHE10501946	CSB-E12935h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.