Human connective tissue growth factor (CTGF) ELISA kit

Human connective tissue growth factor (CTGF) ELISA kit

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Mfr.No.

CSB-E07875h

Alternative Names

connective tissue growth factor, CCN2, HCS24, IGFBP8, MGC102839, NOV2, hypertrophic chondrocyte-specific protein 24, insulin-like growth factor-binding protein 8

Reactivity

Human

Detection Range

3.75 pg/mL-120 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	96	94-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	94
	Range %	90-98
1:4	Average %	99
	Range %	95-104
1:8	Average %	102
	Range %	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.269	2.298	2.284	2.086
5	1.674	1.698	1.686	1.488
2.5	1.081	1.098	1.090	0.892
1.25	0.729	0.769	0.749	0.551
0.625	0.437	0.457	0.447	0.249
0.312	0.388	0.363	0.376	0.178
0.156	0.283	0.303	0.293	0.095
0	0.197	0.198	0.198	?

Species

Homo sapiens

Sensitivity

1.875 pg/mL

Limit Of Detection

450 nm

UniProt ID	P29279
Antigen	Connective Tissue Growth Factor

Applications

ELISA

Research Area

CTGF

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501942	CSB-E07875h	96T	$790	Add to Cart
BHE10501942	CSB-E07875h	5x96T	$3792	Add to Cart
BHE10501942	CSB-E07875h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.