Human Complement fragment 5a,C5a ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08512h

Sensitivity

9.75 pg/ml

Detection Range

39 pg/ml-2500 pg/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human APOH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	91
	Range %	86-95
1:2000	Average %	102
	Range %	97-107
1:4000	Average %	91
	Range %	85-97
1:8000	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	C5a

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Structure and biocompatibility of poly (vinyl alcohol)-based and agarose-based monolithic composites with embedded divinylbenzene-styrene polymeric Berezhna et al,Biomaterials,2013 2:4 Development of biocompatible and safe polyethersulfone hemodialysis membrane incorporated with functionalized multi-walled carbon nanotubes Muhammad Nidzhom Zainol Abidin.et al,Materials Science and Engineering: C,/ Construction and characterization of an antibacterial/anticoagulant dual-functional surface based on poly l-lactic acid electrospun fibrous mats WufengYang.et al,Materials Science and Engineering: C,July 4

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