Human Complement fragment 4b,C4b ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09302h

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Complement fragment 4b,C4b

Recovery

The recovery of rat GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	97
	Range %	92-102
1:10	Average %	98
	Range %	95-103
1:20	Average %	101
	Range %	95-106
1:40	Average %	94
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P0C0L5

Applications	ELISA
Research Area	C4b

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Serum complement C4b, fibronectin, and prolidase are associated with the pathological changes of pulmonary tuberculosis Wang C et al,BMC Infect Dis,2014 Jan 31;14:52. High complement levels in astrocyte-derived exosomes of Alzheimer's disease Goetzl EJ.et al,Ann Neurol,Feb 6

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BHE10501936	CSB-E09302h	10x96T	$6602.50	Add to Cart