Human Complement fragment 3b,C3b ELISA Kit

Mfr.No.

CSB-E09303h

Alternative Names

Complement fragment 3b,C3b

Reactivity

Human

Detection Range

3.12 ng/mL-200 ng/mL

Sample Type

Saliva, Serum, Urine, Cerebrospinal fluid

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-95
EDTA plasma (n=4)	106	103-111

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	92
	Range %	85-99
1:4	Average %	90
	Range %	88-94
1:8	Average %	101
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.088	2.252	2.170	2.080
100	1.585	1.676	1.631	1.541
50	1.163	1.104	1.134	1.044
25	0.831	0.765	0.798	0.708
12.5	0.550	0.524	0.537	0.447
6.25	0.344	0.345	0.345	0.255
3.12	0.182	0.183	0.183	0.093
0	0.090	0.089	0.090	?

Species

Homo sapiens

Sensitivity

0.78 ng/mL

Limit Of Detection

450 nm

Antigen	Complement fragment 3b,C3b

Applications

ELISA

Research Area

C3b

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis. Eriksson CE.et al,J Neuroimmunol.,295-296:130-8. Diminished expression of B2-GPI is associated with a reduced ability to mitigate complement activation in anti-GPIIb/IIIa-mediated immune thrombocytopenia Zhu X.et al,Ann Hematol,Dec 29

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SKU	Supplier No.	Size	Your price
BHE10501931	CSB-E09303h	96T	$695	Add to Cart
BHE10501931	CSB-E09303h	5x96T	$3336	Add to Cart
BHE10501931	CSB-E09303h	10x96T	$6602.50	Add to Cart

Human Complement fragment 3b,C3b ELISA Kit

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