Human Complement fragment 3b,C3b ELISA Kit

Mfr.No.

CSB-E09303h

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Complement fragment 3b,C3b

Recovery

The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-95
EDTA plasma (n=4)	106	103-111

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	92
	Range %	85-99
1:4	Average %	90
	Range %	88-94
1:8	Average %	101
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	C3b

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis. Eriksson CE.et al,J Neuroimmunol.,295-296:130-8. Diminished expression of B2-GPI is associated with a reduced ability to mitigate complement activation in anti-GPIIb/IIIa-mediated immune thrombocytopenia Zhu X.et al,Ann Hematol,Dec 29

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